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DOI:
上海中医药杂志:2015,49(6):87-90
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柑橘不同用药部位中橙皮苷、柠檬苦素及诺米林含量与其体外抗氧化和抗乳腺癌活性相关性研究
李云1,邢丽娜1,周明眉1,裴瑾2,罗静2
(1.上海中医药大学中医方证与系统生物学研究中心(上海201203);2.成都中医药大学药学院中药资源教研室(四川成都610075))
Correlative studies of content of hesperidin, limonin and nomilin in different parts of citrus with their antioxidant and anti-breast cancer cell activities
LI Yun1, XING Li-na1, ZHOU Ming-mei1, PEI Jin2, LUO Jing2
(1.Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine;2.School of Pharmacy, Chengdu University of Traditional Chinese Madicine)
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中文摘要:   目的:考察柑橘不同用药部位——橘核、橘络和陈皮中橙皮苷、柠檬苦素及诺米林的含量与其抗氧化和抗乳腺癌活性的相关性  方法:通过HPLC法检测来自不同产地的橘核、橘络和陈皮药材中橙皮苷、柠檬苦素及诺米林的含量,结合体外DPPH和FRAP抗氧化实验方法以及MTT法研究3种成分的含量差异对药材抗氧化活性和抑制乳腺癌细胞MCF-7增殖作用的影响  结果:橙皮苷含量:陈皮>橘络>橘核;柠檬苦素类物质含量:橘核>橘络>陈皮;体外抗氧化活性:陈皮>橘络>橘核;抑制乳腺癌细胞增殖作用:橘核>橘络>陈皮  结论:橘核、橘络和陈皮的抗氧化活性强弱主要与橙皮苷含量相关,而三者的抗乳腺癌活性强弱主要与其柠檬苦素和诺米林含量相关。
Abstract:  Objective:This paper is to study the correlation of contents of hesperidin,limonin and nomilin from different parts of citrus with their antioxidant and anti-breast cancer cell activities  Methods:The contents of hesperidin,limonin and nomilin in different parts of citrus,tangerine seed,pith and peel were detected with HPLC method. Furthermore,the in vitro antioxidant effects of the three parts of tangerine were studied with DPPH and FRA  Methods:,and anti-breast cancer cell (MCF-7) activities were studied with MTT assay  Results:The tangerine peel has the highest content of hesperidin,while the tangerine seed contains the highest content of limonoids. Relevant to the content study,tangerine peel has the stronger antioxidant activities in vitro than the other two,while the tangerine seed show more stronger anti-breast cancer cell activities  Conclusion:The antioxidant activites of tangerine seed,pith and peel are mainly correlated with their contents of hesperidin,and the anti-breast cancer cell activities are correlated with their contents of limonins.
文章编号:20150631     中图分类号:    文献标志码:
基金项目:国家自然科学基金资助项目(J13100340-13);上海市教委预算内项目(2013JW17);上海中医药大学中药学一流学科创新研究基金资助项目(ZYX-CXYJ-018)
引用文本:
李云,邢丽娜,周明眉,等.柑橘不同用药部位中橙皮苷、柠檬苦素及诺米林含量与其体外抗氧化和抗乳腺癌活性相关性研究[J].上海中医药杂志,2015,49(6):87-90.
LI Yun,XING Li-na,ZHOU Ming-mei,et al.Correlative studies of content of hesperidin, limonin and nomilin in different parts of citrus with their antioxidant and anti-breast cancer cell activities[J].Shanghai J Tradit Chin Med,2015,49(6):87-90.